Comparison of Staphylococcal Flora in Denture Plaque and the Surface of the Pharyngeal Mucous Membrane in Kidney Transplant Recipients

• Published in: Transplantation proceedings Vol. 48; no. 5; pp. 1590 - 1597
• Main Authors: Majchrzak, K., Mierzwinska-Nastalska, E., Chmura, A., Kwiatkowski, A., Paczek, L., Mlynarczyk, G., Szymanek-Majchrzak, K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2016
• Subjects: Anti-Bacterial Agents - pharmacology; Biofilms; Dentures - microbiology; Drug Resistance, Bacterial; Humans; Kidney Transplantation; Microbial Sensitivity Tests; Mucous Membrane - microbiology; Pharynx - microbiology; Staphylococcal Infections - microbiology; Staphylococcus - isolation & purification; Surgery
• ISSN: 0041-1345; 1873-2623; 1873-2623
• DOI: 10.1016/j.transproceed.2016.03.016

Biofilm occurring on removable prostheses is a reservoir of bacterial flora, consisting of both physiological and pathogenic multidrug-resistant microorganisms. Patients undergoing dialysis and organ transplantation are particularly prone to bacterial infections, which can have its source in denture plaque. This study was a comparison of the composition of staphylococcal flora within the surface of the pharyngeal mucous membrane as well as denture plaque in kidney transplant recipients. The study included 44 subjects with removable prostheses who reported for a kidney transplant procedure. Swab samples were collected from prostheses and the pharyngeal wall. Isolated strains were identified and investigated for drug resistance. A total of 72 strains of Staphylococcus were isolated from the denture plaque and 53 from the pharynx. In the pharynx, the following species prevailed: Staphylococcus epidermidis, Staphylococcus warneri, and Staphylococcus aureus. The following species prevailed in denture plaque: S epidermidis, S aureus, and Staphylococcus haemolyticus. Among the pharyngeal strains, antibiotic resistance most commonly referred to natural penicillin (77%), constitutive macrolide-lincosamide-streptogramin B resistance (28%), and tetracycline (26.4%). In case of denture plaque, the highest percentage of strains demonstrated resistance to natural penicillin (60%), fosfomycin (32%), and cefoxitin (25%). In 10 subjects (48%), Staphylococcus-induced infections occurred in the first year after transplantation, 5 of which had the same bacterial strain as cultured previously from dentures. The denture biofilm and surface of the pharynx differ in terms of bacterial composition and bacterial drug resistance profiles. Denture plaque constitutes a considerable reservoir of staphylococcal flora, which can be a potential source of infection in organ transplant recipients. •Staphylococcal flora composition differs on dentures and the pharynx.•Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus haemolyticus most commonly inhabit both niches.•Antibiotic profiles of the same species differ on dentures and the pharynx.•Nearly one-half of isolated strains were multidrug-resistant organisms.