Antifungal Action of Herbal Plants' Glycolic Extracts against Candida Species

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 6; p. 2857
• Main Authors: Meccatti, Vanessa Marques, Santos, Lana Ferreira, de Carvalho, Lara Steffany, Souza, Clara Bulhões, Carvalho, Cláudio Antonio Talge, Marcucci, Maria Cristina, Abu Hasna, Amjad, de Oliveira, Luciane Dias
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.03.2023; MDPI
• Subjects: Acids; Antifungal activity; Antifungal Agents - chemistry; Biofilms; Candida; Candida albicans; Candida dubliniensis; Candida krusei; Candida tropicalis; Cell viability; Coronaviruses; COVID-19; Curcuma longa; Curcumin; Fungal infections; Fungicides; Glycols; Herbal medicine; High performance liquid chromatography; Humans; Hypotheses; Kruskal-Wallis test; Liquid chromatography; Microbial Sensitivity Tests; p-Coumaric acid; Pathogens; Plant extracts; Plant Extracts - chemistry; Punica granatum; Quercetin; Rosa centifolia; Rosmarinus; Severe acute respiratory syndrome coronavirus 2; Variance analysis; Candida albicans; Rosa centifolia; Candida krusei; Curcuma longa; Rosmarinus; Candida tropicalis; Punica granatum; Candida dubliniensis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28062857

spp. cause fungal infection that affects patients' oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of L., L., L., and L. glycolic extracts against , , , and planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey's tests, and by Kruskal-Wallis and Dunn's tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of and glycolic extracts was effective against , and biofilms over different periods ( < 0.05). The combination of and glycolic extracts was effective against and biofilms after 30 min, and against after 24 h, with all combinations showing an average reduction of 50% in cell viability ( < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against spp. in different concentrations and times of action.