Semi-solid-state fermentation: A promising alternative for neomycin production by the actinomycete Streptomyces fradiae

• Published in: Journal of biotechnology Vol. 165; no. 3-4; pp. 195 - 200
• Main Authors: Machado, Isabel, Teixeira, José A., Rodríguez-Couto, Susana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.06.2013; Elsevier; Elsevier BV
• Subjects: Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; biotechnology; Biotechnology - methods; Disk Diffusion Antimicrobial Tests; Fermentation; Glucose - metabolism; Neomycin; Neomycin - metabolism; Neomycin - pharmacology; nylon; Science & Technology; Semi-solid-state fermentation; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Streptomyces - metabolism; Streptomyces fradiae; Submerged fermentation; Submerged fermentation; Semi-solid-state fermentation; Staphylococcus epidermidis; Streptomyces fradiae; Neomycin
• ISSN: 0168-1656; 1873-4863; 1873-4863; 0168-1656
• DOI: 10.1016/j.jbiotec.2013.03.015

•We studied the production of neomycin by Streptomyces fradiae.•Semi-solid-state fermentation led to higher neomycin production than SmF.•The support used greatly influenced the production of neomycin by S. fradiae.•Semi-solid-state fermentation holds great potential for neomycin production. The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum value of 13,903μg/mL on the 10th day of cultivation. As a control, the same experiment was performed under submerged fermentation (SmF) conditions, without solid support. Here the production of neomycin by S. fradiae was about 55-fold lower (i.e. 250μg/mL) than that obtained for SSF.