The impact of Triton WR-1339 induced hyperlipidemia on the effects of benzo(a)pyrene or guaiacol on α- and γ-tocopherol pools and selected markers of pro-/antioxidative balance in rat plasma and erythrocytes

• Published in: Environmental toxicology and pharmacology Vol. 33; no. 3; pp. 386 - 393
• Main Authors: Gawlik, Maciej, Gawlik, Małgorzata B., Brandys, Jerzy
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2012
• Subjects: alpha-tocopherol; alpha-Tocopherol - blood; Animals; Benzo(a)pyrene; Benzo(a)pyrene - toxicity; biotransformation; carcinogenicity; Carcinogens - toxicity; Co-oxygenation; Emergency; Erythrocytes; gamma-tocopherol; gamma-Tocopherol - blood; Guaiacol; Guaiacol - toxicity; Hyperlipidemia; Hyperlipidemias - blood; Hyperlipidemias - chemically induced; lipid peroxidation; Lipid Peroxidation - drug effects; Lipids - blood; Male; males; Malondialdehyde - blood; Polyethylene Glycols; Rat blood; Rats; Rats, Wistar; superoxide dismutase; Superoxide Dismutase - blood; Surface-Active Agents; Tocopherol; Co-oxygenation; Guaiacol; Benzo(a)pyrene; Tocopherol; Rat blood; Hyperlipidemia
• ISSN: 1382-6689; 1872-7077; 1872-7077
• DOI: 10.1016/j.etap.2012.01.004

► The impact of hyperlipidemia induced by Triton WR-1339 on co-oxygenation effects of benzo(a)pyrene (BaP) or guaiacol was studied in rat. ► Tocopherol pools and pro-/antioxidative balance markers in blood were measured. ► In normolipemic groups malondialdehyde (MDA) increased and erythrocyte tocopherol decrease after BaP or guaiacol short-lasting treatment. ► Pro-oxidative effects of BaP and guaiacol were not increased in hyperlipemic animals. The toxicity of carcinogenic benzo(a)pyrene (BaP) can be intensified by the pro-oxidative effects of metabolic activation. The oxidatively active products can be formed during enzymatic biotransformation or in the process of co-oxygenation with lipid peroxidation. This study assesses if the acute hyperlipidemia can increase pro-oxidative effects of BaP as a factor intensifying processes of lipid peroxidation and co-oxygenation. After three days of i.p. administration of BaP or guaiacol (equimolar dose 10mg/kg b.w.) without or with the hyperlipidemia inducer-Triton WR-1339 to male Wistar rats, the levels of α- and γ-tocopherol were measured in erythrocytes and plasma together with the level of lipid peroxidation as malonyldialdehyde (MDA) concentration. Guaiacol was chosen as a reference substance due to its high ability to co-oxygenate. Additionally, the activity of superoxide dismutase (Cu,ZnSOD) in erythrocytes and plasma was monitored. In normolipaemic groups the significant decrease in erythrocyte α-tocopherol pool and the increase in lipid peroxidation level were observed after BaP or guaiacol administration. In hyperlipaemic groups, despite the increase in the level of lipid peroxidation, there were no additional effects in tocopherol pools compared to the normolipaemic groups which could be attributed to co-oxygenation. Decrease of α-tocopherol in erythrocytes was proportional to the reduction in normolipemic subjects when accounting for the migration to hyperlipemic plasma. There was no co-oxygenation effect on the activity of superoxide dismutase (Cu,ZnSOD) in blood.