Assessment of a microplate method for detection of staphylococcal secretory inhibitor of platelet microbicidal protein

Abstract We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in ≤24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and c...

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Bibliographic Details
Published inDiagnostic microbiology and infectious disease Vol. 63; no. 1; pp. 118 - 120
Main Authors Ivanov, Iuri B, Gritsenko, Viktor A
Format Journal Article
LanguageEnglish
Russian
Published New York, NY Elsevier Inc 01.01.2009
Elsevier
Subjects
Bacterial Proteins - pharmacology
Bacteriological Techniques - methods
beta-Thromboglobulin - antagonists & inhibitors
Blood Bactericidal Activity - drug effects
Blood Platelets - metabolism
Chronic Disease
Humans
Infectious Disease
Internal Medicine
Male
Platelet microbicidal protein
Prostate - microbiology
Prostatitis - microbiology
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry - methods
Staphylococcal Infections - microbiology
Staphylococci
Staphylococcus - metabolism
Staphylococci
Platelet microbicidal protein
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Summary:Abstract We developed a novel microplate spectrophotometric assay for the detection of staphylococcal secretory inhibitor of platelet microbicidal protein (SIPMP) in ≤24 h. The levels of SIPMP production agreed with the results obtained by viable counting method. Both methods may be acceptable and cheap, allowing for their routine use in clinical laboratories.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2008.09.011