Purification of Stearidonic Acid (18:4(n-3)) and Hexadecatetraenoic Acid (16:4(n-3)) from Algal Fatty Acid with Lipase and Medium Pressure Liquid Chromatography

• Published in: Bioscience, biotechnology, and biochemistry Vol. 64; no. 11; pp. 2454 - 2457
• Main Authors: ISHIHARA, Kenji, MURATA, Masakazu, KANENIWA, Masaki, SAITO, Hiroaki, KOMATSU, Wataru, SHINOHARA, Kazuki
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.11.2000; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: Biological and medical sciences; Biotechnology; Chlorophyta - chemistry; Chromatography, Gas; Chromatography, Liquid; Fatty Acids, Nonesterified - chemistry; Fatty Acids, Omega-3 - chemistry; Fatty Acids, Omega-3 - isolation & purification; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - isolation & purification; Food industries; Fundamental and applied biological sciences. Psychology; hexadecatetraenoic acid; lipase; Lipase - chemistry; marine alga; Methods. Procedures. Technologies; Others; Phaeophyceae - chemistry; Research and development. New food products, dietetic foods and beverages; selective esterification; Stearidonic acid; Ulva pertusa; Undaria pinnatifida; Various methods and equipments; Medium pressure; Purification; Enzyme; Algae; Polyunsaturated fatty acid; Triacylglycerol lipase; Esterases; Liquid chromatography; Selectivity; Method; Esterification; Carboxylic ester hydrolases; Edible alga; Marine environment; Heterokontophyta; Chlorophyta; Preconcentration; Hydrolases; Phaeophyceae; Thallophyta; Enzymatic reaction
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.64.2454

Stearidonic acid (18:4(n-3)) and hexadecatetraenoic acid (16:4(n-3)) are included in some edible marine algae such as Undaria pinnatifida and Ulva pertusa with relatively high compositions (up to 40%) of total fatty acids. In order to prepare 16:4(n-3) and 18:4(n-3) enriched fatty acid concentrates, we screened for a suitable lipase which concentrates these acids by the removal of other fatty acids in the selective esterification reaction reported by Shimada et al. (Shimada et al. (1997), J. Am. Oil Chem. Soc., 74, 1465-1470). In combination with the lipase reaction and reversed-phase medium pressure liquid chromatography, we purified 18:4(n-3) and 16:4(n-3) to more than 95% purity.