Development of molecular markers based on real-time PCR to detect flax and sesame in commercial amaranth products

• Published in: Food science and biotechnology Vol. 33; no. 14; pp. 3313 - 3322
• Main Authors: Kim, Yeon Mi, Jang, Cheol Seong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.11.2024; Springer Nature B.V; 한국식품과학회
• Subjects: allergenicity; Amaranth; Amaranthus; Anaphylaxis; anti-allergic agents; Chemistry; Chemistry and Materials Science; Contaminants; Deoxyribonucleic acid; DNA; Flax; Food allergies; Food Science; genetic markers; linseed; Linum usitatissimum; Nutrition; oligodeoxyribonucleotides; Peptides; Polymerase chain reaction; Public health; quantitative polymerase chain reaction; Real time; Research Article; Sesamum indicum; 식품과학; SYBR® Green real-time PCR; DNA marker; Linum usitatissimum; Sesamum indicum; Amaranthus
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01584-2

Amaranthus , Sesamum indicum , and Linum usitatissimum are the most popular oilseed grains worldwide. Protein-rich Amaranthus contains bioactive peptides, is nutritious, and exhibits anti-allergic properties. Sesamum indicum is a primary trigger of anaphylaxis. Linum usitatissimum also displays allergenic properties. A DNA marker assessable using quantitative real-time PCR was developed to detect S. indicum and L. usitatissimum as allergenic contaminants of anti-allergenic Amaranthus . The efficiency of each primer set ranged from 90–98%, and high linear correlation (R 2  > 0.99) was obtained between crossover values and the log DNA concentration. We established a Ct value of 0.1% of the binary as a cutoff. The practical application of the designed marker was confirmed by analyzing 20 commercial products. The qPCR system developed for detecting flaxseed and sesame can be applied for regulatory monitoring of allergenic substances in commercial amaranth-containing foods, thus contributing to protecting public health and safety.