Protein-based lateral flow assays for COVID-19 detection

Abstract To combat the enduring and dangerous spread of COVID-19, many innovations to rapid diagnostics have been developed based on proteinprotein interactions of the SARS-CoV-2 spike and nucleocapsid proteins to increase testing accessibility. These antigen tests have most prominently been develop...

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Bibliographic Details
Published inProtein engineering, design and selection Vol. 34
Main Authors Mahmoudinobar, Farbod, Britton, Dustin, Montclare, Jin Kim
Format Journal Article
LanguageEnglish
Published England Oxford University Press 15.02.2021
Subjects
Animals
Antibodies, Viral - analysis
Antibodies, Viral - immunology
Antigens, Viral - analysis
Antigens, Viral - immunology
Coronavirus Nucleocapsid Proteins - analysis
Coronavirus Nucleocapsid Proteins - immunology
COVID-19 - diagnosis
COVID-19 - immunology
COVID-19 Serological Testing - instrumentation
COVID-19 Serological Testing - methods
Equipment Design
Humans
Phosphoproteins - analysis
Phosphoproteins - immunology
Point-of-Care Testing
SARS-CoV-2 - immunology
SARS-CoV-2 - isolation & purification
Short Review
Spike Glycoprotein, Coronavirus - analysis
Spike Glycoprotein, Coronavirus - immunology
covid-19
diagnostics
antibody test
lateral flow assay
antigen test
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Summary:Abstract To combat the enduring and dangerous spread of COVID-19, many innovations to rapid diagnostics have been developed based on proteinprotein interactions of the SARS-CoV-2 spike and nucleocapsid proteins to increase testing accessibility. These antigen tests have most prominently been developed using the lateral flow assay (LFA) test platform which has the benefit of administration at point-of-care, delivering quick results, lower cost, and does not require skilled personnel. However, they have gained criticism for an inferior sensitivity. In the last year, much attention has been given to creating a rapid LFA test for detection of COVID-19 antigens that can address its high limit of detection while retaining the advantages of rapid antibodyantigen interaction. In this review, a summary of these proteinprotein interactions as well as the challenges, benefits, and recent improvements to protein based LFA for detection of COVID-19 are discussed.
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Farbod Mahmoudinobar and Dustin Britton contributed equally to this work.
ISSN:1741-0126
1741-0134
1741-0134
DOI:10.1093/protein/gzab010