Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms and renal cancer risk

• Published in: Lipids in health and disease Vol. 14; no. 1; p. 143
• Main Authors: Liu, ZhiHong, Xiao, YingMing, Tang, LiangYou, Jiang, Liang, Wang, YuJie, Zhang, RuoChen, Wei, Qiang, Lu, YiPing
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.11.2015; BioMed Central
• Subjects: Adult; Analysis; Apolipoprotein A-I - genetics; Apolipoproteins; Carcinoma, Renal cell; Carcinoma, Renal Cell - genetics; Carcinoma, Renal Cell - secondary; Care and treatment; Case-Control Studies; Complications and side effects; Disease susceptibility; Female; Gene Frequency; Genetic Association Studies; Genetic polymorphisms; Genetic Predisposition to Disease; Genotype; Humans; Influence; Kidney Neoplasms - genetics; Kidney Neoplasms - pathology; Lymphatic Metastasis; Male; Middle Aged; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; United States
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/s12944-015-0132-0

Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. The objective of the present study was to evaluate the association of two genetic variants (-75 G/A and +83 C/T) of APOA1 with predisposition to renal cancer. A total of 432 subjects, including 216 pathologically-proven renal cancer cases and 216 age- and gender-matched healthy controls, were recruited into this hospital-based case-control study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing. Patients with renal cancer had a significantly higher frequency of APOA1 -75 AA genotype [odds ratio (OR) = 2.10, 95% confidence interval (CI) = 1.18, 3.75; P = 0.01] and APOA1 -75 A allele (OR =1.40, 95% CI = 1.05, 1.87; P = 0.02) than controls. When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04). This study is, to our knowledge, the first to examine prospectively an increased risk role of APOA1 -75 AA genotype and APOA1 -75 A allele in renal cancer susceptibility.