Deferoxamine regulates neuroinflammation and iron homeostasis in a mouse model of postoperative cognitive dysfunction

• Published in: Journal of neuroinflammation Vol. 13; no. 1; p. 268
• Main Authors: Li, Yuping, Pan, Ke, Chen, Lin, Ning, Jiao-Lin, Li, Xiaojun, Yang, Ting, Terrando, Niccolò, Gu, Jianteng, Tao, Guocai
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.10.2016; BioMed Central
• Subjects: Aging; Animals; Cell Line, Transformed; Cognition disorders; Cognition Disorders - drug therapy; Cognition Disorders - etiology; Cognition Disorders - prevention & control; Conditioning (Psychology) - drug effects; Deferoxamine; Deferoxamine - therapeutic use; Disease Models, Animal; Encephalitis - drug therapy; Encephalitis - etiology; Encephalitis - pathology; Encephalitis - prevention & control; Fear; Health aspects; Homeostasis - drug effects; Influence; Iron - metabolism; Laparotomy - adverse effects; Male; Maze Learning - drug effects; Mice; Mice, Inbred C57BL; Microglia - drug effects; Microglia - metabolism; Oxidative Stress - drug effects; Postoperative Complications - drug therapy; Postoperative Complications - physiopathology; Risk factors; Siderophores - therapeutic use; Time Factors; China; Iron; Cytokines; Surgery; Hippocampus; Microglia
• ISSN: 1742-2094; 1742-2094
• DOI: 10.1186/s12974-016-0740-2

Postoperative cognitive dysfunction (POCD) is a common complication after surgery, especially amongst elderly patients. Neuroinflammation and iron homeostasis are key hallmarks of several neurological disorders. In this study, we investigated the role of deferoxamine (DFO), a clinically used iron chelator, in a mouse model of surgery-induced cognitive dysfunction and assessed its neuroprotective effects on neuroinflammation, oxidative stress, and memory function. A model of laparotomy under general anesthesia and analgesia was used to study POCD. Twelve to 14 months C57BL/6J male mice were treated with DFO, and changes in iron signaling, microglia activity, oxidative stress, inflammatory cytokines, and neurotrophic factors were assessed in the hippocampus on postoperative days 3, 7, and 14. Memory function was evaluated using fear conditioning and Morris water maze tests. BV2 microglia cells were used to test the anti-inflammatory and neuroprotective effects of DFO. Peripheral surgical trauma triggered changes in hippocampal iron homeostasis including ferric iron deposition, increase in hepcidin and divalent metal transporter-1, reduction in ferroportin and ferritin, and oxidative stress. Microglia activation, inflammatory cytokines, brain-derived neurotropic factor impairments, and cognitive dysfunction were found up to day 14 after surgery. Treatment with DFO significantly reduced neuroinflammation and improved cognitive decline by modulating p38 MAPK signaling, reactive oxygen species, and pro-inflammatory cytokines release. Iron imbalance represents a novel mechanism underlying surgery-induced neuroinflammation and cognitive decline. DFO treatment regulates neuroinflammation and microglia activity after surgery.