Highly Specific Peptide-Mediated Cuvette-Form Localized Surface Plasmon Resonance (LSPR)-Based Fipronil Detection in Egg

Herein, we have developed peptide-coated gold nanoparticles (AuNPs) based on localized surface plasmon resonance (LSPR) sensor chips that can detect fipronil with high sensitivity and selectivity. The phage display technique has been exploited for the screening of highly specific fipronil-binding pe...

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Published inBiosensors (Basel) Vol. 12; no. 11; p. 914
Main Authors Yoo, Jingon, Han, Soobin, Park, Bumjun, Sonwal, Sonam, Alhammadi, Munirah, Kim, Eunsu, Aliya, Sheik, Lee, Eun-Seon, Jeon, Tae-Joon, Oh, Mi-Hwa, Huh, Yun Suk
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 23.10.2022
MDPI
Subjects
Analysis
biosensor
Biosensors
Chip formation
Chromatography
Cost analysis
E coli
Eggs
Fabrication
Fipronil
Food
Glass substrates
Gold
Insecticides
localized surface plasmon resonance (LSPR)
Mass production
Nanoparticles
Onsite
peptide screening
Peptides
Pesticides
Phage display
Phages
Polyethylene glycol
Reaction time
Resonance
Scanning electron microscopy
Selectivity
Sensitivity
Sensors
Surface plasmon resonance
South Korea
United States > US
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Summary:Herein, we have developed peptide-coated gold nanoparticles (AuNPs) based on localized surface plasmon resonance (LSPR) sensor chips that can detect fipronil with high sensitivity and selectivity. The phage display technique has been exploited for the screening of highly specific fipronil-binding peptides for the selective detection of the molecule. LSPR sensor chips are fabricated initially by attaching uniformly synthesized AuNPs on the glass substrate, followed by the addition of screened peptides. The parameters, such as the peptide concentration of 20 µg mL−1 and the reaction time of 30 min, are further optimized to maximize the efficacy of the fabricated LSPR sensor chips. The sensing analysis is performed systematically under standard fipronil solutions and spike samples from eggs. The developed sensor has shown excellent sensitivity towards both standard solutions and spike samples with limit of detection (LOD) values of 0.01 ppb, respectively. Significantly, the developed LSPR sensor chips offer distinct features, such as a facile fabrication approach, on-site sensing, rapid analysis, cost-effectiveness, and the possibility of mass production, in which the chips can be effectively used as a promising and potential on-site detection tool for the estimation of fipronil.
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These authors contributed equally to this work.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12110914