Fungal Biomass Protein Production from Trichoderma harzianum Using Rice Polishing

• Published in: BioMed research international Vol. 2017; no. 2017; pp. 1 - 9
• Main Authors: Rajoka, Muhammad I., Arshad, Muhammad, Mustafa, Ghulam, Ahmed, Sibtain
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2017; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Agriculture; Amino acids; Animal Feed; Animals; Biomass; Biotechnology; Biotechnology industry; Carbon - chemistry; Carbon - metabolism; Cellulose - chemistry; Cellulose - metabolism; Colleges & universities; Feeds; Fermentation; Fungal Proteins - biosynthesis; Fungal Proteins - chemistry; Fungi; Grinding and polishing; Methods; Microorganisms; Nitrogen - chemistry; Nitrogen - metabolism; Oryza - chemistry; Poultry; Proteins; Rice; Trichoderma - chemistry; Trichoderma - metabolism; Yeast; Pakistan
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2017/6232793

Industrially important enzymes and microbial biomass proteins have been produced from fungi for more than 50 years. High levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. The aim of this study was to access the potential of Trichoderma harzianum to produce fungal biomass protein from rice polishings. Maximum biomass yield was obtained at 5% (w/v) rice polishings after 72 h of incubation at 28°C at pH 4. Carbon and nitrogen ratio of 20 : 1 gave significantly higher production of fungal biomass protein. The FBP in the 75 L fermenter contained 49.50% crude protein, 32.00% true protein, 19.45% crude fiber, 9.62% ash, 11.5% cellulose content, and 0.325% RNA content. The profile of amino acids of final FBP exhibited that all essential amino acids were present in great quantities. The FBP produced by this fungus has been shown to be of good nutritional value for supplementation to poultry. The results presented in this study have practical implications in that the fungus T. harzianum could be used successfully to produce fungal biomass protein using rice polishings.