Suberoylanilide hydroxamic acid (SAHA) inhibits transforming growth factor-beta 2-induced increases in aqueous humor outflow resistance

• Published in: The Journal of biological chemistry Vol. 297; no. 3; p. 101070
• Main Authors: Fujimoto, Tomokazu, Inoue-Mochita, Miyuki, Iraha, Satoshi, Tanihara, Hidenobu, Inoue, Toshihiro
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2021; American Society for Biochemistry and Molecular Biology
• Subjects: Akt; bone morphogenetic protein (BMP); extracellular matrix protein; extracellular signal-regulated kinase (ERK); eye; histone deacetylase inhibitor (HDAC inhibitor); phosphatase and tensin homolog (PTEN); transforming growth factor beta (TGF-β); HTM; extracellular matrix protein; PHLPP1; MTM; PTEN; histone deacetylase inhibitor (HDAC inhibitor); SC; α-SMA; transforming growth factor beta (TGF-β); PI3K; RT-PCR; MSC; SAHA; PDPK1; phosphatase and tensin homolog (PTEN); bone morphogenetic protein (BMP); Akt; TDP-A; BMP7; extracellular signal-regulated kinase (ERK); ECM; eye; ZO-1; PIK3CA; TEER; TM; TGF-β2; ERK; HDAC
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/j.jbc.2021.101070

Transforming growth factor-beta 2 (TGF-β2) is highly concentrated in the aqueous humor of primary open-angle glaucoma patients. TGF-β2 causes fibrosis of outflow tissues, such as the trabecular meshwork (TM), and increases intraocular pressure by increasing resistance to aqueous humor outflow. Recently, histone deacetylase (HDAC) activity was investigated in fibrosis in various tissues, revealing that HDAC inhibitors suppress tissue fibrosis. However, the effect of HDAC inhibitors on fibrosis in the eye was not determined. Here, we investigated the effect of suberoylanilide hydroxamic acid (SAHA), an HDAC inhibitor, on TGF-β2-induced increased resistance to aqueous humor outflow. We found that SAHA suppressed TGF-β2-induced outflow resistance in perfused porcine eyes. Moreover, SAHA cotreatment suppressed TGF-β2-induced ocular hypertension in rabbits. The permeability of monkey TM (MTM) and Schlemm’s canal (MSC) cell monolayers was decreased by TGF-β2 treatment. SAHA inhibited the effects of TGF-β2 on the permeability of these cells. TGF-β2 also increased the expression of extracellular matrix proteins (fibronectin and collagen type I or IV) in MTM, MSC, and human TM (HTM) cells, while SAHA inhibited TGF-β2-induced extracellular matrix protein expression in these cells. SAHA also inhibited TGF-β2-induced phosphorylation of Akt and ERK, but did not inhibit Smad2/3 phosphorylation, the canonical pathway of TGF-β signaling. Moreover, SAHA induced the expression of phosphatase and tensin homolog, a PI3K/Akt signaling factor, as well as bone morphogenetic protein 7, an endogenous antagonist of TGF-β. These results imply that SAHA prevents TGF-β2-induced increases in outflow resistance and regulates the non-Smad pathway of TGF-β signaling in TM and MSC cells.