Antiulcer Activity of Anthraquinone-Flavonoid Complex of Rumex tianschanicus Losinsk

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 5; p. 2347
• Main Authors: Seitimova, Gulnaz A, Shokan, Aksholpan K, Tolstikova, Tatyana G, Zhukova, Nataliya A, Korulkin, Dmitriy Yu, Kudrina, Nataliya O, Litvinenko, Yuliya A, Meduntseva, Nataliya D, Terletskaya, Nina V, Kulmanov, Timur E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.03.2023; MDPI
• Subjects: Animals; Anthraquinone; Anthraquinones; Anthraquinones - chemistry; Anti-Ulcer Agents - chemistry; antiulcer effect; Chronic illnesses; Chrysophanic acid; Column chromatography; Composition; Disease; Emodin; Epithelium; Ethanol; flavonoid; Flavonoids; Flavonoids - therapeutic use; Folk medicine; Hemodynamics; Herbal medicine; Indomethacin; Infrared spectroscopy; Laboratory animals; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Medicine; Metabolites; NMR; NMR spectroscopy; Nuclear magnetic resonance; Phytochemicals; Plant Extracts - chemistry; Polyphenols; Quercetin; Rats; Roots; Rumex; Rumex - chemistry; Rumex tianschanicus Losinsk; Stomach; stomach ulcer; Stomach Ulcer - chemically induced; Thin layer chromatography; Ulcers; anthraquinone; stomach ulcer; antiulcer effect; flavonoid; Rumex tianschanicus Losinsk
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28052347

The composition of an ethanol extract from the roots of Losinsk of the Trans-Ili Alatau wild flora was studied in order to determine its antiulcer activity. The phytochemical composition of the anthraquinone-flavonoid complex from (AFC) revealed the presence of numerous polyphenolic compounds, the most abundant of which are anthraquinones (1.77%), flavonoids (6.95%), and tannins (13.39%). The use of column chromatography (CC) and thin-layer chromatography (TLC) in conjunction with UV, IR, NMR spectroscopy, and mass spectrometry data allowed the researchers to isolate and identify the major components of the anthraquinone-flavonoid complex's polyphenol fraction: physcion, chrysophanol, emodin, isorhamnetin, quercetin, and myricetin. The gastroprotective effect of the polyphenolic fraction of the anthraquinone-flavonoid complex (AFC) of roots was examined in an experimental model of rat gastric ulcer induced by indomethacin. The preventive and therapeutic effect of the anthraquinone-flavonoid complex at a dose of 100 mg/kg was analyzed using intragastric administration per day for 1 to 10 days, followed by a histological examination of stomach tissues. It has been demonstrated that prophylactic and prolonged use of the AFC in laboratory animals resulted in significantly less pronounced hemodynamic and desquamative changes in the epithelium of gastric tissues. The acquired results thus offer fresh insight into the anthraquinone and flavonoid metabolite component composition of roots, and they imply that the examined extract can be used to develop herbal medicines with antiulcer activity.