Antigen Specificity in Subsets of Mucous Membrane Pemphigoid

• Published in: Journal of investigative dermatology Vol. 126; no. 12; pp. 2631 - 2636
• Main Authors: Rashid, Khwaja A., Gürcan, Hakan M., Razzaque Ahmed, A.
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.12.2006; Nature Publishing; Elsevier Limited
• Subjects: Antibody Specificity; Autoantibodies; Autoantibodies - blood; Biological and medical sciences; Bullous diseases of the skin; Dermatology; Epitopes; Eye Diseases - immunology; Fluorescent Antibody Technique, Indirect; Gingiva; Humans; Immunoblotting; Integrin alpha6 - immunology; Integrin beta4 - immunology; Integrins; Internet; Laminin; Longitudinal Studies; Medical sciences; Mouth Diseases - immunology; Mucous Membrane; ocular cicatricial pemphigoid; Pemphigoid; Pemphigoid, Benign Mucous Membrane - immunology; Pemphigoid, Bullous - immunology; Bullous dermatosis; Immunopathology; Skin disease; Scarring pemphigoid; Stomatology; Dermatology; Autoimmune disease; Conjunctiva disease; Antigen; Eye disease; Specificity; Oral cavity disease; Mucosa disease
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1038/sj.jid.5700465

Mucous membrane pemphigoid (MMP) has several subsets based on target antigens recognized by their sera. MMP and ocular cicatricial pemphigoid (OCP) sera recognize β4 integrin subunit, oral pemphigoid sera recognize α6 integrin subunit, and anti-epiligrin cicatricial pemphigoid sera recognize laminin 5. Our aim is to determine if autoantibodies in the sera of patients with MMP, OCP, and oral pemphigoid (OP) recognize only their target antigens, and to see if this specificity is maintained throughout the clinical course. An immunoblot assay using bovine gingival lysate was used as substrate. Fifteen MMP patients, eight with OCP, and 15 OP patients were studied before therapy and at multiple intervals during the clinical course. Absorption and blocking studies were performed to determine binding specificity. Sera of patients with MMP and OCP recognize only β4 integrin subunit, and sera of OP patients recognize α6 integrin throughout the clinical course. The sera of patients in the subsets of MMP described in this report show adherence and selectivity to target antigen during the entire clinical course, without crossover, interaction, or change. Hence, these subsets of MMP provide an excellent model to study clinical correlation with antigen and antibody specificity, in autoimmunity.