Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

• Published in: Journal of Clinical Microbiology Vol. 44; no. 7; pp. 2601 - 2604
• Main Authors: Rogers, G.B, Carroll, M.P, Serisier, D.J, Hockey, P.M, Jones, G, Kehagia, V, Connett, G.J, Bruce, K.D
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2006
• Subjects: Adult; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacterial Infections - microbiology; Bacteriology; Cluster Analysis; Cystic Fibrosis - complications; Cystic Fibrosis - microbiology; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Genes, rRNA; Humans; Lung - microbiology; Mouth - microbiology; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S - genetics; Sputum - microbiology

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical sampl...