Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical sampl...

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Published inJournal of Clinical Microbiology Vol. 44; no. 7; pp. 2601 - 2604
Main Authors Rogers, G.B, Carroll, M.P, Serisier, D.J, Hockey, P.M, Jones, G, Kehagia, V, Connett, G.J, Bruce, K.D
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.07.2006
Subjects
Adult
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Infections - microbiology
Bacteriology
Cluster Analysis
Cystic Fibrosis - complications
Cystic Fibrosis - microbiology
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Genes, rRNA
Humans
Lung - microbiology
Mouth - microbiology
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sputum - microbiology
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Summary:The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.
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Corresponding author. Mailing address: King's College London, Pharmaceutical Science Research Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom. Phone: 44 020 7848 4670. Fax: 44 020 7848 4500. E-mail: kenneth.bruce@kcl.ac.uk.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.02282-05