Chemical constitution and effect of extracts of tomato plants byproducts on the enteric viral surrogates

• Published in: International journal of environmental health research Vol. 25; no. 3; pp. 299 - 311
• Main Authors: Silva-Beltrán, Norma Patricia, Ruiz-Cruz, Saul, Chaidez, Cristobal, Ornelas-Paz, José de Jesús, López-Mata, Marco A., Márquez-Ríos, Enrique, Estrada, Maria Isabel
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 04.05.2015; Taylor & Francis LLC
• Subjects: Antiviral Agents - chemistry; Antiviral Agents - pharmacology; bacteriophages; Bacteriophages - drug effects; Chlorogenic Acid - analysis; Chromatography, High Pressure Liquid - methods; Coumaric Acids - analysis; Cultivars; Effects; extracts; Gallic Acid - analysis; Levivirus - drug effects; Lycopersicon esculentum; Lycopersicon esculentum - chemistry; Phenols; Phenols - analysis; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; polyphenols; Quercetin - analysis; Rutin - analysis; tomato byproducts; Tomatoes; polyphenols; extracts; tomato byproducts; bacteriophages
• ISSN: 0960-3123; 1369-1619
• DOI: 10.1080/09603123.2014.938030

Byproducts of tomato are known to include phenolic compounds but have not been studied in depth. In this study, the phenolic compositions of (stem, leaf, root, and whole plant) of two tomato cultivars, Pitenza and Floradade, were analyzed by HPLC-DAD. In parallel, the antiviral effects of crude extracts on viral surrogates, the bacteriophages MS2 and Av-05 were evaluated. The leaf extracts from the two varieties showed the highest concentration of phenolic compounds. The compounds identified were gallic acid, chlorogenic acid, ferulic acid, cafeic acid, rutin, and quercetin, and they represented 3174.3 and 1057.9 mg/100 g dried weight of the Pitenza and Floradade cultivars, respectively. MS2 and Av-05 titers at 5 mg/mL were reduced by 3.47 and 5.78 log 10 PFU/mL and 3.78 and 4.93 log 10 PFU/mL by Pitenza and Floradade cultivar leaf extract, respectively. These results show that tomato extracts are natural sources of bioactive substances with antiviral activity.