Applications of DNA shuffling to pharmaceuticals and vaccines

DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve genes. Single and multigene traits that require many mutations for improved phenotypes can be evolved rapidly. DNA shuffling technology has been...

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Bibliographic Details
Published inCurrent opinion in biotechnology Vol. 8; no. 6; pp. 724 - 733
Main Authors Patten, Phillip A, Howard, Russell J, Stemmer, Willem PC
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.12.1997
Subjects
Amino Acid Sequence
Biological Evolution
Chemistry, Pharmaceutical
DNA - chemistry
Enzymes - chemistry
Genetic Therapy
Genetic Vectors
Humans
Molecular Sequence Data
Recombinant Proteins - chemistry
Recombination, Genetic
Sequence Homology, Amino Acid
Vaccines
Viruses - genetics
MLV murine leukemia virus
IFN interferon
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Summary:DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve genes. Single and multigene traits that require many mutations for improved phenotypes can be evolved rapidly. DNA shuffling technology has been significantly enhanced in the past year, extending its range of applications to small molecule pharmaceuticals, pharmaceutical proteins, gene therapy vehicles and transgenes, vaccines and evolved viruses for vaccines, and laboratory animal models.
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ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(97)80127-9