A contiguous Not I restriction map of band q22.3 of human chromosome 21

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 89; no. 8; pp. 3222 - 3226
• Main Authors: Wang, D, Fang, H, Cantor, C R, Smith, C L
• Format: Journal Article
• Language: English
• Published: United States National Acad Sciences 15.04.1992; National Academy of Sciences
• Subjects: Animals; Base Sequence; Cell Line; CHO Cells; Chromosome Banding; Chromosomes, Human, Pair 21; Cricetinae; Deoxyribonucleases, Type II Site-Specific; Deoxyribonucleic acid; DNA; DNA - genetics; DNA - isolation & purification; Genetic Linkage; Genetics; Humans; Mice; Molecular Sequence Data; Polymorphism, Genetic; restriction fragment length polymorphism; Restriction Mapping; Telomere - physiology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.8.3222

A contiguous high-resolution NotI restriction map of the distal region of the long arm of human chromosome 21 was constructed by three strategies: linking clones to identify adjacent pieces of DNA, partial digestion to identify neighboring fragments, and cell line polymorphisms to prove identity or adjacency of DNA fragments. Twenty-nine single-copy DNA probes and five linking clone probes were used to determine the order of 30 Not I fragments, covering 10 megabases of DNA in band q22.3. Smaller Not I fragments occur preferentially in this region, suggesting that band q22.3 is unusually rich in genes, since Not I sites occur almost exclusively in CpG islands. Comparison of the physical map and genetic maps in this region reveals a 10-fold higher than average recombination frequency.