Comparison of different culture media and growth conditions for recognition of Arcanobacterium haemolyticum

• Published in: Diagnostic microbiology and infectious disease Vol. 61; no. 2; pp. 232 - 234
• Main Authors: García-de-la-Fuente, Celia, Campo-Esquisabel, Ana Belén, Unda, Fabián, Ruiz de Alegría, Carlos, Benito, Natividad, Martínez-Martínez, Luis
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.06.2008; Elsevier
• Subjects: Actinomycetaceae - isolation & purification; Actinomycetaceae - physiology; Animals; Arcanobacterium haemolyticum; Bacteriological Techniques - methods; Bacteriology; Biological and medical sciences; Culture Media; Fundamental and applied biological sciences. Psychology; Hemolysis; Horse blood; Horses; Infectious Disease; Infectious diseases; Internal Medicine; Medical sciences; Microbiology; Miscellaneous; Pharyngitis; Time Factors; Trypticase soy agar with 5; β-Hemolysis; β-Hemolysis; Trypticase soy agar with 5; Pharyngitis; Horse blood; Microbiology; Blood; Infection; Growing medium; Vertebrata; Mammalia; Arcanobacterium haemolyticum; Horse; Bacteria; ENT disease; Pharynx disease; Perissodactyla; Veterinary; Ungulata; Recognition
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2008.01.020

Abstract Detection of Arcanobacterium haemolyticum is based upon typical β-hemolysis and colony morphology, but it may go undetected if only conventional sheep blood agar media for detection of β-hemolytic streptococci are used. The influence of different culture media, atmospheres, and times of incubation for the recognition of colonies of 47 isolates of A. haemolyticum was studied. After 48 h of incubation, trypticase soy agar with 5% horse blood in 5% CO2 was the best medium.