MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells

• Published in: Journal of biomedical research Vol. 24; no. 6; pp. 436 - 443
• Main Authors: Li, Ping, Lu, Xiaoming, Wang, Yingyi, Sun, Lihua, Qian, Chunfa, Yan, Wei, Liu, Ning, You, Yongping, Fu, Zhen
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.11.2010; Editorial Department of Journal of Biomedical Research
• Subjects: chemoresistance; glioma stem cells; miR-181b; PCR检测; proliferation; Research Paper; 小分子RNA; 干细胞; 生长抑制率; 细胞增殖; 脑胶质瘤; 集落形成; miR-181b; chemoresistance; proliferation; glioma stem cells
• ISSN: 1674-8301
• DOI: 10.1016/S1674-8301(10)60058-9

MicroRNAs regulate self renewal and differentiation of cancer stem cells.There,we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study.MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting.After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide,secondary neurosphere assay,soft agar colony assay and MTT assay were performed.Compared with U87 cells,the expression of miR-181b was significantly decreased in U87 glioma stem cells.Overexpression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar,and the cell growth inhibition rates increased in a time-dependent manner in U87 glioma stem cells infected with miR-181b lentivirus.Furthermore,miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony,and on cell growth inhibition rates.MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to temozolomide in glioma stem cells.