Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 12
• Main Authors: Mazzola, Salvatore, Colombo, Paolo, Cuttitta, Angela, Tagliavia, Marcello, Salamone, Monica, Bonura, Angela, Bennici, Carmelo, Vlah, Sara, Masullo, Tiziana, Longo, Alessandra, Bulati, Matteo, Nicosia, Aldo
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016; John Wiley & Sons, Inc
• Subjects: Anemonia viridis; Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - isolation & purification; Apoptosis; Cancer; Cell Extracts - administration & dosage; Cell Extracts - isolation & purification; Cell growth; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells; Cytotoxicity; Dose-Response Relationship, Drug; Humans; Morphology; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - pathology; Peptides; Proteins; Sea Anemones - chemistry; Treatment Outcome; Tumors; Venom; Water quality; Italy
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2016/3849897

In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cytotoxic activity by means of erythrocyte haemolysis test, MTS, and LDH assays. Finally, the antiproliferative activities of three of these fractions were studied on PC3, PLC/PRF/5, and A375 human cancer cell lines. Our analysis revealed that the four fractions showed different protein contents and diverse patterns of activity towards human PBMC and cancer cell lines. Interestingly, fractions III and IV exerted cytotoxic effects on human cells. Conversely, fractions I and II displayed very low toxic effects associated with antiproliferative activities on cancer cell lines.