Differentiating agents regulate cathepsin B gene expression in HL-60 cells

• Published in: Journal of leukocyte biology Vol. 66; no. 4; pp. 609 - 616
• Main Authors: Berquin, Isabelle M., Yan, Shiqing, Katiyar, Kamna, Huang, Li, Sloane, Bonnie F., Troen, Bruce R.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.10.1999
• Subjects: Alitretinoin; Butyrates - pharmacology; calcitriol; Calcitriol - pharmacology; cathepsin B; Cathepsin B - genetics; Cell Differentiation - drug effects; ctsb gene; Cycloheximide - pharmacology; Dactinomycin - pharmacology; Gene Expression Regulation - drug effects; granulocyte; HL-60 Cells; Humans; Mitogens - pharmacology; monocyte; mRNA; Nucleic Acid Synthesis Inhibitors - pharmacology; posttranscriptional regulation; Promoter Regions, Genetic; Protein Synthesis Inhibitors - pharmacology; RNA, Messenger; sodium butyrate; Tetradecanoylphorbol Acetate - pharmacology; Transcription, Genetic; transcriptional regulation; Tretinoin - pharmacology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.66.4.609

We utilized HL‐60 cells as a model system to examine the regulation of ctsb gene expression by differentiating agents. Inducers of monocytic differentiation [phorbol ester (PMA), calcitriol (D3), and sodium butyrate (NaB)] and inducers of granulocytic differentiation [all‐trans retinoic acid (RA) and 9‐cis retinoic acid (9‐cis RA)] increase ctsb mRNA levels in a dose‐dependent manner as determined by Northern blot hybridization. D3 and retinoids exert additive effects, suggesting that these agents act in part through distinct pathways. Actinomycin D decay experiments indicate that D3, NaB, RA, and 9‐cis RA do not alter mRNA stability. In contrast, PMA markedly increases the half‐life of ctsb mRNA. In transient transfection assays, PMA and NaB both stimulate transcription of the luciferase reporter gene placed under the control of ctsb promoter fragments. Thus, inducers of HL‐60 cell differentiation can regulate the expression of the ctsb gene at both transcriptional and posttranscriptional levels. J. Leukoc. Biol. 66: 609–616; 1999.