Differentiating agents regulate cathepsin B gene expression in HL-60 cells

We utilized HL‐60 cells as a model system to examine the regulation of ctsb gene expression by differentiating agents. Inducers of monocytic differentiation [phorbol ester (PMA), calcitriol (D3), and sodium butyrate (NaB)] and inducers of granulocytic differentiation [all‐trans retinoic acid (RA) an...

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Published inJournal of leukocyte biology Vol. 66; no. 4; pp. 609 - 616
Main Authors Berquin, Isabelle M., Yan, Shiqing, Katiyar, Kamna, Huang, Li, Sloane, Bonnie F., Troen, Bruce R.
Format Journal Article
LanguageEnglish
Published United States Society for Leukocyte Biology 01.10.1999
Subjects
Alitretinoin
Butyrates - pharmacology
calcitriol
Calcitriol - pharmacology
cathepsin B
Cathepsin B - genetics
Cell Differentiation - drug effects
ctsb gene
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Gene Expression Regulation - drug effects
granulocyte
HL-60 Cells
Humans
Mitogens - pharmacology
monocyte
mRNA
Nucleic Acid Synthesis Inhibitors - pharmacology
posttranscriptional regulation
Promoter Regions, Genetic
Protein Synthesis Inhibitors - pharmacology
RNA, Messenger
sodium butyrate
Tetradecanoylphorbol Acetate - pharmacology
Transcription, Genetic
transcriptional regulation
Tretinoin - pharmacology
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Summary:We utilized HL‐60 cells as a model system to examine the regulation of ctsb gene expression by differentiating agents. Inducers of monocytic differentiation [phorbol ester (PMA), calcitriol (D3), and sodium butyrate (NaB)] and inducers of granulocytic differentiation [all‐trans retinoic acid (RA) and 9‐cis retinoic acid (9‐cis RA)] increase ctsb mRNA levels in a dose‐dependent manner as determined by Northern blot hybridization. D3 and retinoids exert additive effects, suggesting that these agents act in part through distinct pathways. Actinomycin D decay experiments indicate that D3, NaB, RA, and 9‐cis RA do not alter mRNA stability. In contrast, PMA markedly increases the half‐life of ctsb mRNA. In transient transfection assays, PMA and NaB both stimulate transcription of the luciferase reporter gene placed under the control of ctsb promoter fragments. Thus, inducers of HL‐60 cell differentiation can regulate the expression of the ctsb gene at both transcriptional and posttranscriptional levels. J. Leukoc. Biol. 66: 609–616; 1999.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.66.4.609