Gene expression profiling of diffuse large B‐cell lymphoma supervised by CD21 expression

• Published in: British journal of haematology Vol. 142; no. 4; pp. 562 - 570
• Main Authors: Miyazaki, Kana, Yamaguchi, Motoko, Suguro, Miyuki, Choi, Woonyoung, Ji, Yuan, Xiao, Lianchun, Zhang, Wei, Ogawa, Shoko, Katayama, Naoyuki, Shiku, Hiroshi, Kobayashi, Tohru
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.2008; Blackwell
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Biomarkers, Tumor - metabolism; CD21; diffuse large B‐cell lymphoma; Female; Gene Expression Profiling; Gene Ontology; Hematologic and hematopoietic diseases; Humans; immunoglobulin M; Immunohistochemistry; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma, Large B-Cell, Diffuse - genetics; Lymphoma, Large B-Cell, Diffuse - immunology; Male; Medical sciences; Microarray Analysis; Middle Aged; Receptors, Complement 3d - metabolism; Hematology; immunoglobulin M; B cell neoplasm; Malignant hemopathy; diffuse large B-cell lymphoma; IgM; Gene expression profile; Gene; Lymphoproliferative syndrome; Genetics; Diffuse large B cell lymphoma; Complement receptor type 2; gene expression profiling; Gene Ontology; Cancer; CD21
• ISSN: 0007-1048; 1365-2141
• DOI: 10.1111/j.1365-2141.2008.07218.x

Summary This study investigated the gene expression profiles of 40 cases of diffuse large B‐cell lymphoma (DLBCL) according to CD21 expression, a favourable prognostic factor in DLBCL. Signature genes were analysed by Gene Ontology Tree Machine, and genes concerned with the immune system and related categories were significantly upregulated in CD21− DLBCLs. Of 40 DLBCLs, four were germinal centre B cell‐like (GCB) and 36 non‐GCB. Of the 36 non‐GCB DLBCLs, 14 CD21+ DLBCLs showed significantly better overall survival than the 22 CD21− DLBCLs (P = 0·036). Hierarchical cluster analysis of signature genes related to CD21 was applied to previously published data sets, resulting in two groups for each data set, CD21+ type DLBCLs and CD21− type DLBCLs. Survival of CD21+ type DLBCLs was significantly better than that of CD21– type (P = 0·006 and P = 0·004, respectively). In both data sets, CD21+ type DLBCLs predominantly included GCB DLBCLs compared with CD21− type. The top classifier gene of CD21 expression was IGHM, and the five of nine Gene Ontology categories significant in CD21– DLBCLs included IGHM. Immunohistochemical analysis of 216 DLBCLs confirmed that overall survival of surface (s) IgM+ DLBCLs was significantly poorer than that of sIgM‐ DLBCLs (P = 0·013).