Effects of moderate- and high-intensity chronic exercise on brain-derived neurotrophic factor expression in fast and slow muscles

• Published in: Muscle & nerve Vol. 53; no. 3; pp. 446 - 451
• Main Authors: Jiménez-Maldonado, Alberto, Cerna-Cortés, Joel, Castro-Rodríguez, Elena M., Montero, Sergio A., Muñiz, Jesús, Rodríguez-Hernández, Alejandrina, Lemus, Mónica, De Álvarez-Buylla, Elena Roces
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.03.2016; Wiley Subscription Services, Inc
• Subjects: Analysis of Variance; Animals; BDNF expression; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - genetics; Brain-Derived Neurotrophic Factor - metabolism; chronic exercise; Epilepsy; gene; Gene Expression Regulation - physiology; Male; Muscle Contraction - physiology; Muscle, Skeletal - physiology; Physical Conditioning, Animal; Physical Endurance - physiology; Proteins; Rats; Rats, Wistar; RNA, Messenger - metabolism; Rodents; skeletal muscle; BDNF expression; brain-derived neurotrophic factor; skeletal muscle; chronic exercise; gene
• ISSN: 0148-639X; 1097-4598
• DOI: 10.1002/mus.24757

ABSTRACT Introduction: Brain‐derived neurotrophic factor (BDNF) protein expression is sensitive to cellular activity. In the sedentary state, BDNF expression is affected by the muscle phenotype. Methods: Eighteen Wistar rats were divided into the following 3 groups: sedentary (S); moderate‐intensity training (MIT); and high‐intensity training (HIT). The training protocol lasted 8 weeks. Forty‐eight hours after training, total RNA and protein levels in the soleus and plantaris muscles were obtained. Results: In the plantaris, the BDNF protein level was lower in the HIT than in the S group (P < 0.05). A similar effect was found in the soleus (without significant difference). In the soleus, higher Bdnf mRNA levels were found in the HIT group (P < 0.001 vs. S and MIT groups). In the plantaris muscle, similar Bdnf mRNA levels were found in all groups. Conclusions: These results indicate that high‐intensity chronic exercise reduces BDNF protein level in fast muscles and increases Bdnf mRNA levels in slow muscles. Muscle Nerve 53: 446–451, 2016