The temporal profile of retinal cell genesis in the marmoset monkey

• Published in: Journal of comparative neurology (1911) Vol. 524; no. 6; pp. 1193 - 1207
• Main Authors: Hendrickson, A., Possin, D., Kwan, W.C., Huang, J., Bourne, J.A.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 15.04.2016; Wiley Subscription Services, Inc
• Subjects: Age Factors; Animals; Callithrix; Callithrix jacchus; eye; Female; glia; Macaca; Male; Neurogenesis - physiology; neuron; nonhuman primate; Retina - cytology; Retina - growth & development; Retinal Cone Photoreceptor Cells - physiology; Retinal Rod Photoreceptor Cells - physiology; RRID: AB_ 10000344; RRID: AB_10000347; RRID: AB_2298772; RRID: AB_2313756; RRID: AB_2314215; RRID: AB_2314227; RRID: AB_2315274; thymidine analogs; Time Factors; Callithrix jacchus; neuron; RRID: AB_ 10000344; RRID: AB_2315274; nonhuman primate; RRID: AB_10000347; glia; RRID: AB_2314227; eye; RRID: AB_2298772; RRID: AB_2314215; thymidine analogs; RRID: AB_2313756
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.23897

ABSTRACT The New World marmoset monkey (Callithrix jacchus) has a relatively short gestational period compared with other primates but possesses a retina at a similar stage of maturation by birth. Previous studies have highlighted that the complex fovea of the marmoset undergoes a more rapid postnatal development in comparison with the Macaca monkey, reaching a mature stage earlier than these species. In this current study, we examined the prenatal proliferation profile of cells in the entire retina employing the thymidine analogs and also determined their phenotype by double‐label immunocytochemistry using type‐specific markers. Akin to other primate species, we demonstrate a centroperipheral gradient in the emergence of both neurons and Müller glia with cones, ganglion cells, and horizontal cells generated first in the fovea at fetal day (Fd)70–74 and with the last generated at the retinal edge at Fd115. Rods, bipolar cells, amacrine cells, displaced amacrine cells, and Müller glia were generated between Fd76 and Fd135 along the same gradient. Similar to foveal development, marmoset neuronal generation was rapid, only taking 51% of gestation whereas in Macaca this takes 81%. J. Comp. Neurol. 524:1193–1207, 2016. © 2015 Wiley Periodicals, Inc. In this study, the authors profiled the prenatal proliferation of cells in the entire retina of the New World marmoset (Callithrix jacchus) by employing the thymidine analogs, and also determined their phenotype postnatally by double‐label immunocytochemistry using type‐specific markers. Akin to other nonhuman primates, a centroperipheral gradient of development was observed but with a more rapid completion.