Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor

• Published in: American journal of reproductive immunology (1989) Vol. 75; no. 2; pp. 112 - 125
• Main Authors: Yoneda, Noriko, Yoneda, Satoshi, Niimi, Hideki, Ueno, Tomohiro, Hayashi, Shirou, Ito, Mika, Shiozaki, Arihiro, Urushiyama, Daichi, Hata, Kenichiro, Suda, Wataru, Hattori, Masahira, Kigawa, Mika, Kitajima, Isao, Saito, Shigeru
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01.02.2016; Wiley Subscription Services, Inc
• Subjects: Adult; Amniotic Fluid - immunology; Amniotic Fluid - microbiology; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacterial Infections - immunology; Bacterial Infections - microbiology; Chorioamnionitis - immunology; Chorioamnionitis - microbiology; Coinfection - immunology; Coinfection - microbiology; DNA, Bacterial - analysis; DNA, Fungal - analysis; Eukaryote-made thermostable DNA polymerase; Female; Fungi - genetics; Fungi - isolation & purification; Humans; Infections; Inflammation; Interleukin-8 - immunology; intra-amniotic infection; Leukocyte Count; Microorganisms; Mycoplasma; Mycoses - immunology; Mycoses - microbiology; Obstetric Labor, Premature - immunology; Obstetric Labor, Premature - microbiology; polymerase chain reaction; Polymerase Chain Reaction - methods; polymicrobial infection; Pregnancy; preterm labor; Prognosis; RNA, Ribosomal, 16S - genetics; Ureaplasma; Ureaplasma spp; Young Adult; preterm labor; Eukaryote-made thermostable DNA polymerase; intra-amniotic infection; polymicrobial infection; polymerase chain reaction; Ureaplasma spp
• ISSN: 1046-7408; 1600-0897; 1600-0897
• DOI: 10.1111/aji.12456

Problem To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra‐amniotic (IA) inflammation using a highly sensitive and reliable PCR‐based method. Method of Study To detect prokaryotes using a nested PCR‐based method, eukaryote‐made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. Results The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL‐8 levels and a significantly shorter amniocentesis‐to‐delivery interval. Conclusions Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.