Interaction of enterohemorrhagic Escherichia coli O157:H7 with mouse intestinal mucosa

In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10⁹ and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical att...

Full description

Saved in:
Bibliographic Details
Published inFEMS microbiology letters Vol. 283; no. 2; pp. 196 - 202
Main Authors Girard, Francis, Frankel, Gad, Phillips, Alan D, Cooley, William, Weyer, Ute, Dugdale, Alexandra H.A, Woodward, Martin J, La Ragione, Roberto M
Format Journal Article
LanguageEnglish
Published Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.06.2008
Blackwell Publishing Ltd
Blackwell
Oxford University Press
Subjects
Actin
Animals
Bacteria
Bacterial Adhesion
Bacteriology
Biological and medical sciences
colonization
E coli
EHEC O157:H7
Epithelium
Escherichia coli
Escherichia coli O157 - physiology
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Female
Fundamental and applied biological sciences. Psychology
Ileum - microbiology
Intestinal Mucosa - microbiology
Intestine
Lesions
Mice
Mice, Inbred ICR
Microbiology
Miscellaneous
Mucosa
Pathogens
Polymerization
Proteins
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Rodents
Type III secretion system
Type III secretion system
colonization
EHEC O157:H7
Digestive system
Colibacillosis
Escherichia coli
Mucosa
Rodentia
Gut
Infection
Vertebrata
Mammalia
Mouse
Bacteriosis
Bacteria
Enterobacteriaceae
Online AccessGet full text

Cover

More Information
Summary:In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10⁹ and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical attaching and effacing (A/E) lesions were seen, albeit infrequently. While the effector protein Tir was essential for A/E lesion formation, the bacterial type III secretion system adaptor protein TccP was dispensable. These results suggest that A/E lesions on mouse intestinal mucosa can be formed independently of robust actin polymerization.
Bibliography:http://dx.doi.org/10.1111/j.1574-6968.2008.01166.x
Editor: Masao Mitsuyama
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01166.x