Molecular sexing of Jungle crow (Corvus macrorhynchos japonensis) and Carrion crow (Corvus corone corone) using a feather

A molecular sexing method using the intron-size difference between chromo-helicase-DNA binding protein (CHD1) genes on the Z and W sex chromosomes (CHD1Z and CHD1W, respectively) was developed for wild Jungle crows and wild Carrion crows. The polymerase chain reaction product sizes were 661 bp for C...

Full description

Saved in:
Bibliographic Details
Published inAnimal science journal Vol. 79; no. 2; pp. 158 - 162
Main Authors Fukui, E.(Utsunomiya Univ. (Japan)), Sugita, S, Yoshizawa, M
Format Journal Article
LanguageEnglish
Published Melbourne, Australia Blackwell Publishing Asia 01.04.2008
Blackwell Publishing Ltd
Subjects
ADN
Animal reproduction
Birds
Carrion crow
CORBEAU
CROWS
CUERVO
DIAGNOSTICO DEL SEXO
DNA
feather
FEATHERS
Females
Genes
Jungle crow
Males
METHODE
METHODS
METODOS
Molecular biology
molecular sexing
PCR
PLUMAS
PLUME
SEX DIAGNOSIS
SEXAGE
Online AccessGet full text

Cover

More Information
Summary:A molecular sexing method using the intron-size difference between chromo-helicase-DNA binding protein (CHD1) genes on the Z and W sex chromosomes (CHD1Z and CHD1W, respectively) was developed for wild Jungle crows and wild Carrion crows. The polymerase chain reaction product sizes were 661 bp for CHD1Z and 465 bp for CHD1W in both the Jungle and the Carrion crows. Each male crow sample produced a single fragment (CHD1Z), whereas female samples produced two fragments (CHD1Z and CHD1W), which differed by 200 bp in crows. In the present study, sexing live Jungle and Carrion crows was performed by applying the method to a feather for molecular sexing.
Bibliography:U40
L01
2008004680
ArticleID:ASJ512
istex:891E958CDAC50647B95C8E1B4003FEEE572EA367
ark:/67375/WNG-PV9GJJSR-2
ISSN:1344-3941
1740-0929
DOI:10.1111/j.1740-0929.2008.00512.x