Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers, and the Antioxidant and Antibacterial Capacity of the Extract

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 1; p. 113
• Main Authors: Zhang, Henghui, Wang, Xiaoli, He, Dongliang, Zou, Dongliang, Zhao, Runzhu, Wang, Huifang, Li, Shuying, Xu, Yongping, Abudureheman, Buhailiqiemu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.12.2021; MDPI
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; antibacterial activity; Antibacterial agents; antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Chemical Fractionation - methods; Chinese medicine; Design of experiments; Design techniques; Dose-Response Relationship, Drug; Ethanol; Flavonoids; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; Flowers; Flowers - chemistry; Food industry; Medical research; Microbial Sensitivity Tests; Optimization; Pharmaceutical industry; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Response surface methodology; Rutin; Sapindaceae - chemistry; Scavenging; Seeds; Sulfonic acid; Temperature; total flavonoids; ultrasound-assisted extraction; Xanthoceras sorbifolia Bunge; United States > US; China; antibacterial activity; Xanthoceras sorbifolia Bunge; total flavonoids; response surface methodology; ultrasound-assisted extraction; antioxidant activity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27010113

In the present work, the extraction process of total flavonoids (TFs) from flowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid-liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities on , and . Therefore, flowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.