UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

MicroRNAs （miRNAs） are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B （UVB） radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 （PTEN） expression in HaCaT cells through an unknow...

Full description

Saved in:
Bibliographic Details
Published inJournal of biomedical research Vol. 25; no. 2; pp. 135 - 140
Main Authors Li, Wei, Di, Wu, Hua, Lijuan, Zhou, Bingrong, Guo, Ze, Luo, Dan
Format Journal Article
LanguageEnglish
Published China Elsevier B.V 01.03.2011
Editorial Department of Journal of Biomedical Research
Subjects
apoptosis
HaCaT cells
HaCaT细胞
MicroRNA-141
miRNAs
PTEN
Research Paper
UVB
基因表达水平
小分子RNA
异硫氰酸荧光素
非编码RNA
UVB
PTEN
apoptosis
MicroRNA-141
HaCaT cells
Online AccessGet full text

Cover

More Information
Summary:MicroRNAs （miRNAs） are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B （UVB） radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 （PTEN） expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucleotide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
Bibliography:MicroRNAs （miRNAs） are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B （UVB） radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 （PTEN） expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucleotide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
32-1810/R
MicroRNA-141, PTEN, UVB, apoptosis, HaCaT cells
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
The authors reported no conflict of interests.
ISSN:1674-8301
DOI:10.1016/S1674-8301(11)60017-1