Is there a relationship between phosphatidylinositol trisphosphate and F-actin polymerization in human neutrophils?

• Published in: The Journal of biological chemistry Vol. 265; no. 28; pp. 16725 - 16728
• Main Authors: Eberle, M, Traynor-Kaplan, A E, Sklar, L A, Norgauer, J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.10.1990; American Society for Biochemistry and Molecular Biology
• Subjects: 550201 - Biochemistry- Tracer Techniques; ACTIN; Actins - blood; ANIMALS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; Cell physiology; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; ESTERS; Fundamental and applied biological sciences. Psychology; Humans; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LEUKOCYTES; Light; LIGHT NUCLEI; LIPIDS; Macromolecular Substances; MAMMALS; MAN; MATERIALS; MEMBRANE PROTEINS; Miscellaneous; Molecular and cellular biology; NEUTROPHILS; Neutrophils - metabolism; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHATES; Phosphates - blood; PHOSPHOLIPIDS; Phospholipids - blood; PHOSPHORUS 32; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; POLYMERIZATION; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RECEPTORS; SCATTERING; Scattering, Radiation; TRACER TECHNIQUES; VERTEBRATES; Human; Phosphatidylinositol; Phosphoinositide; Neutrophile; Light scattering; Actins; Microfilament; Polymerization; Dose activity relation
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)44818-6

Stimulation of human neutrophils with the chemoattractant N-formyl peptide caused rapid polymerization of F-actin as detected by right angle light scatter and 7-nitrobenz-2-oxa-1,3-diazol (NBD)-phallacidin staining of F-actin. After labeling neutrophils with 32P, exposure to N-formyl peptide induced a fast decrease of phosphatidylinositol 4-bisphosphate (PIP)2, a slow increase of phosphatidic acid, and a rapid rise of phosphatidylinositol 4-trisphosphate (PIP3). Formation of PIP3 as well as actin polymerization was near maximal at 10 s after stimulation. Half-maximal response and PIP3 formation at early time points resulted from stimulation of neutrophils with 0.01 nM N-formyl peptide or occupation of about 200 receptors. Sustained elevation of PIP3, prolonged right angle light scatter response, and F-actin formation required higher concentrations of N-formyl peptide, occupation of thousands of receptors, and high binding rates. When ligand binding was interrupted with an antagonist, F-actin rapidly depolymerized, transient light scatter response recovered immediately, and elevated [32P]PIP3 levels decayed toward initial values. However, recovery of [32P]PIP2 was not influenced by the antagonist. Based on the parallel time courses and dose response of [32P] PIP3, the right angle light scatter response, and F-actin polymerization, PIP3 is more likely than PIP2 to be involved in modulation of actin polymerization and depolymerization in vivo.