Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ

• Published in: Molecules (Basel, Switzerland) Vol. 21; no. 5; p. 602
• Main Authors: Xie, Shu-Wu, Li, Guo-Ting, Qu, Li-Juan, Cao, Yang, Wang, Qian, Zhou, Jie-Yun, Zhong, Rui-Hua, Guo, Xiang-Jie, Zhu, Yan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2016; MDPI
• Subjects: 5-alpha Reductase Inhibitors - therapeutic use; Androgens; Animals; Birth control; Body Fluids - metabolism; contraceptives; drug targets; dutasteride; Dutasteride - therapeutic use; Enzyme-Linked Immunosorbent Assay; Epididymis - metabolism; Fertility; Gene expression; Gene Expression Profiling; Infertility; infertility therapy; Infertility, Male - drug therapy; Infertility, Male - metabolism; iTRAQ; luminal fluids; Male; Motility; Organ Size - drug effects; Protein expression; Proteins; Proteins - physiology; Proteomics; rat epididymis; Rats; Sperm; Sperm Maturation - physiology; China; dutasteride; rat epididymis; proteomics; drug targets; iTRAQ; luminal fluids; contraceptives; infertility therapy

Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provi...