Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ

• Published in: Molecules (Basel, Switzerland) Vol. 21; no. 5; p. 602
• Main Authors: Xie, Shu-Wu, Li, Guo-Ting, Qu, Li-Juan, Cao, Yang, Wang, Qian, Zhou, Jie-Yun, Zhong, Rui-Hua, Guo, Xiang-Jie, Zhu, Yan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.05.2016; MDPI
• Subjects: 5-alpha Reductase Inhibitors - therapeutic use; Androgens; Animals; Birth control; Body Fluids - metabolism; contraceptives; drug targets; dutasteride; Dutasteride - therapeutic use; Enzyme-Linked Immunosorbent Assay; Epididymis - metabolism; Fertility; Gene expression; Gene Expression Profiling; Infertility; infertility therapy; Infertility, Male - drug therapy; Infertility, Male - metabolism; iTRAQ; luminal fluids; Male; Motility; Organ Size - drug effects; Protein expression; Proteins; Proteins - physiology; Proteomics; rat epididymis; Rats; Sperm; Sperm Maturation - physiology; China; dutasteride; rat epididymis; proteomics; drug targets; iTRAQ; luminal fluids; contraceptives; infertility therapy
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21050602

Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provide potential epididymal targets for new contraceptives and infertility treatment. Male rats were treated with dutasteride for 28 consecutive days. We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The confidence of proteome data was validated by enzyme-linked immunosorbent assays. 1045 proteins were tested, and 23 of them presented different expression profiling in the infertile and normal rats. The seven proteins were down-regulated, and 16 proteins were up-regulated. Among the seven proteins which were significantly down-regulated by dutasteride in the epididymal luminal fluids, there were three β-defensins (Defb2, Defb18 and Defb39), which maybe the key proteins involved in epididymal sperm maturation and male fertility. We report for the first time that dutasteride influences the protein expression profiling in the epididymal luminal fluids of rats, and this result provides some new epididymal targets for male contraception and infertility therapy.