Effect of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7

• Published in: Asian Pacific journal of tropical medicine Vol. 9; no. 9; pp. 876 - 880
• Main Authors: Lei, Chang-Jiang, Yao, Chun, Li, De-Ke, Long, Zhi-Xiong, Li, Yuan, Tao, Dan, Liou, Yan-Ping, Zhang, Jiang-Zhou, Liu, Ning
• Format: Journal Article
• Language: English
• Published: India 01.09.2016
• Subjects: cancer;Proliferation;Apoptosis; MiR-520c-3p;MiR-132;Liver; Liver cancer; Proliferation; MiR-132; miR-520c-3p; Apoptosis
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/j.apjtm.2016.07.015

Objective:To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.Methods:Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132 respectively or together.The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry,and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR.Results:Compared with the control group,the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly,and the apoptosis ratio increased distinctly(P<0.05).Besides,the affect of the co-transfection group was better than that of the single transfection group.The protein levels of GPC3(Glypican-3) and YAP(Yes-associated protein),the target genes transfected only by miR-520c-3p and miR-132,respectively,reduced obviously(P<0.05),which was similar with the co-infected cells,but cells transfected by miR-132 only showed a decrease of YAP.Conclusions:The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP,enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically.