Phenolic Profile, Antioxidant, Anti-Enzymatic and Cytotoxic Activity of the Fruits and Roots of Eleutherococcus senticosus (Rupr. et Maxim.) Maxim

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 17; p. 5579
• Main Authors: Graczyk, Filip, Gębalski, Jakub, Makuch-Kocka, Anna, Gawenda-Kempczyńska, Dorota, Ptaszyńska, Aneta A, Grzyb, Sebastian, Bogucka-Kocka, Anna, Załuski, Daniel
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.08.2022; MDPI
• Subjects: Acids; Adaptogens; Antioxidants; Antioxidants - chemistry; Chloroform; Coronaviruses; COVID-19; Cytotoxicity; Dietary supplements; Eleutherococcus - chemistry; Eleutherococcus senticosus; Endangered & extinct species; Endangered species; Extracellular matrix; Flavonoids; Flavonoids - analysis; Fruit - chemistry; Fruits; hyaluronidase; Influenza; intractum; Medical research; Methanol - analysis; Mixtures; Naringenin; Pandemics; Phenolic acids; Phenols; Phenols - analysis; Phytochemicals; Plant Extracts - chemistry; Pneumonia; Polyphenols; Polyphenols - analysis; Popularity; Roots; Severe acute respiratory syndrome coronavirus 2; Tumor cell lines; Tyrosinase; Poland; United States > US; Union of Soviet Socialist Republics > USSR; Europe; China; Russia; COVID-19; hyaluronidase; polyphenols; intractum; tyrosinase; Eleutherococcus senticosus; cytotoxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27175579

(Rupr. et Maxim.) Maxim. is well-known for its adaptogenic properties in traditional Eastern medicine. It has been categorized as an endangered species due to the over-exploitation of the roots. As a result, alternatives must be found, including the usage of renewable aerial parts such as fruits. The goal of this research was to determine the phenolic compounds and the enzymatic, antioxidant, and cytotoxic activities of the intractum gained from the fruits and the mixture of chloroform-methanol roots extract with naringenin (3:7:5). The obtained results showed, that the intractum contained 1.02 mg/g ext. of polyphenols, 0.30 mg/g ext. of flavonoids, and 0.19 mg/g ext. of phenolic acids. In turn, the mixture of chloroform-methanol roots extract with naringenin (3:7:5) contained 159.27 mg/g ext. of polyphenols, 137.47 mg/g ext. of flavonoids, and 79.99 mg/g ext. of phenolic acids. Regarding the anti-enzymatic assay, the IC values for tyrosinase and hyaluronidase were equal to 586.83 and 217.44 [μg/mL] for the intractum, and 162.56 and 44.80 [μg/mL] for the mixture, respectively. Both preparations have possessed significant antioxidant activity in the ABTS, DPPH, and ferrozine tests. No cytotoxic effect on the FaDu and HEP G2 cancer cell lines was observed. Our findings support the traditional use of fruits and roots. Moreover, the results indicate also that adaptogens are rather nontoxic for normal and cancer cells, which corresponds with some hypotheses on adaptogens activity.