Laboratory and field enzyme-immunoassays for diazinon and their application to residue analysis in lanolin, water, and fruit juice

• Published in: Bulletin of environmental contamination and toxicology Vol. 59; no. 3; pp. 375 - 382
• Main Authors: Beasely, H.L. (CSIRO Plant Industry, North Ryde, NSW, Australia.), McAdam, D.P, Larkin, K.A, Ferguson, B.S, Bushway, R.J, Skerritt, J.H
• Format: Journal Article
• Language: English
• Published: New York, NY Springer-Verlag 01.09.1997; Springer Nature B.V
• Subjects: AGUA; Analysis; Analysis methods; Applied sciences; Beverages - analysis; Binding, Competitive; Biological and medical sciences; DIAZINON; Diazinon - analysis; EAU; Exact sciences and technology; Food industries; Fresh Water - analysis; Fruit; FRUIT JUICES; Fundamental and applied biological sciences. Psychology; Fungicides; General pharmacology; Immunoassays; Immunoenzyme Techniques; INSECTICIDAS; INSECTICIDE; INSECTICIDES; Insecticides - analysis; JUGO DE FRUTAS; JUS DE FRUITS; Lanolin - analysis; LANOLINA; LANOLINE; Medical sciences; Natural water pollution; Non alcoholic beverage industries and mineral waters; Pesticide Residues - analysis; Pharmacology. Drug treatments; Pollution; Reproducibility of Results; RESIDU; RESIDUES; RESIDUOS; WATER; Water treatment and pollution; Trace analysis; Water; Chemical analysis; Insecticide; Pesticides; Well; Foodstuff; Organic thiophosphate; Enzyme immunoassay; Fruit juice; Diazinon; Analysis method; Residue; Surface water; Lanolin; Organophosphorus compounds; Water pollution; Quantitative analysis; Chemical contamination
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s001289900488

Sensitive and robust immunoassays were developed for diazinon based on polyclonal antibodies. Diazinon haptens were produced by a 4-stage process and coupled with 3 carrier proteins. Rabbit antisera were raised from the immunogens and the antibodies assessed in a solid-phase antigen assay using Maxisorp 96-microwell plates. After overnight incubation, washing and blocking of the remaining active sites, the colorimetric end point was achieved by addition of hydrogen peroxide-3,3',5,5'-tetramethylbenzidine or diazinon peroxidase. A field test carried out in polystyrene tubes was also developed. Analyses in a variety of media produced no false positives and correlated acceptably with results by gas chromatography. Little or no clean up or sample concentration was required.