Interaction with Soil Bacteria Affects the Growth and Amino Acid Content of Piriformospora indica

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 3; p. 572
• Main Authors: Leyva-Rojas, Jorge A, Coy-Barrera, Ericsson, Hampp, Rüdiger
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.01.2020; MDPI
• Subjects: Alanine; Alanine transaminase; Amino acid composition; amino acid content; Amino acids; Amino Acids - analysis; Azotobacter; Azotobacter - pathogenicity; Bacteria; Basidiomycota - chemistry; Basidiomycota - growth & development; Carbon sources; Discriminant analysis; Endophytes; endophytic fungi; Experiments; Fungi; Glutamate-ammonia ligase; Glutamine; Glycolysis; Influence; Metabolism; Metabolomics; Microorganisms; Morphology; mycorrhiza helper bacteria; Nitrogen; Oxidative Stress; Perspex; Piriformospora indica; Rhizosphere; Rhizosphere microorganisms; Soil bacteria; Soil investigations; Soil Microbiology; Soil microorganisms; Soils; Streptomyces; Streptomyces - pathogenicity; Supervised Machine Learning; Symbiosis; Volatiles; amino acid content; mycorrhiza helper bacteria; Piriformospora indica; endophytic fungi
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25030572

Exploration of the effect of soil bacteria on growth and metabolism of beneficial root endophytic fungi is relevant to promote favorable associations between microorganisms of the plant rhizosphere. Hence, the interaction between the plant-growth-promoting fungus and different soil bacteria was investigated. The parameters studied were fungal growth and its amino acid composition during the interaction. Fungus and bacteria were confronted in dual cultures in Petri dishes, either through agar or separated by a Perspex wall that only allowed the bacterial volatiles to be effective. Fungal growth was stimulated by , whereas AcH 1003 inhibited it and sp. Nov AcH 505 had no effect. To analyze amino acid concentration data, targeted metabolomics was implemented under supervised analysis according to fungal-bacteria interaction and time. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model clearly discriminated - and - interactions, according to the respective score plot in comparison to the control. The most observable responses were in the glutamine and alanine size groups: While AcH 1003 increased the amount of glutamine, decreased it. The fungal growth and the increase of alanine content might be associated with the assimilation of nitrogen in the presence of glucose as a carbon source. The -fixing bacterium should stimulate fungal amino acid metabolism via glutamine synthetase-glutamate synthase (GS-GOGAT). The data pointed to a stimulated glycolytic activity in the fungus observed by the accumulation of alanine, possibly via alanine aminotransferase. The responses toward the growth-inhibiting AcH 1003 suggest an (oxidative) stress response of the fungus.