A Herbal Formula HT048, Citrus unshiu and Crataegus pinnatifida, Prevents Obesity by Inhibiting Adipogenesis and Lipogenesis in 3T3-L1 Preadipocytes and HFD-Induced Obese Rats

• Published in: Molecules (Basel, Switzerland) Vol. 20; no. 6; pp. 9656 - 9670
• Main Authors: Lee, Yoon Hee, Kim, Young-Sik, Song, Mikyung, Lee, Minsu, Park, Juyeon, Kim, Hocheol
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.05.2015; MDPI
• Subjects: 3T3-L1 Cells; Adipocytes; Adipogenesis - drug effects; Animals; anti-obesity; Anti-Obesity Agents - pharmacology; Body fat; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Differentiation - drug effects; Citrus - chemistry; Citrus unshiu; Crataegus - chemistry; Crataegus pinnatifida; Diet; Diet, High-Fat; Drug dosages; Fatty acids; Gene Expression Regulation; Glycerol; Glycerol - metabolism; herbal combination; HT048; Insulin resistance; Lactones - pharmacology; Lipid Metabolism - drug effects; Lipids; Lipogenesis - drug effects; Lipoproteins; Male; Mice; Obesity; Obesity - etiology; Obesity - genetics; Obesity - metabolism; Obesity - prevention & control; Orlistat; Plant Extracts - chemistry; Plant Extracts - pharmacology; PPAR gamma - genetics; PPAR gamma - metabolism; Proteins; Rats; Rats, Sprague-Dawley; Signal Transduction; Triglycerides - metabolism; Crataegus pinnatifida; Citrus unshiu; HT048; anti-obesity; herbal combination
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules20069656

HT048 is a combination composed of Crataegus pinnatifida leaf and Citrus unshiu peel extracts. This study aimed to investigate potential anti-obesity effect of the combination. The 3T3-L1 adipocytes were treated with different doses of HT048 and triglyceride accumulation, glycerol release and adipogenesis-related genes were analyzed. For in vivo study, male Sprague Dawley rats were divided according to experimental diets: the chow diet group, the high-fat diet (HFD) group, the HFD supplemented with orlistat group, the HFD supplemented with HT048 group (0.2% or 0.4%) for 12 weeks. We measured the body weight, serum lipid levels and the expression of genes involved lipid metabolism. HT048 treatment dose-dependently suppressed adipocyte differentiation and stimulated glycerol release. The expressions of PPARγ and C/EBPα mRNA were decreased by HT048 treatment in adipocytes. HT048 supplementation significantly reduced the body and fat weights in vivo. Serum lipid levels were significantly lower in the HT048 supplemented groups than those of the HFD group. Expression of the hepatic lipogenesis-related genes were decreased and expression of the β-oxidation-related genes were increased in rats fed HT048 compared to that of animals fed HFD. These results suggest that HT048 has a potential benefit in preventing obesity through the inhibition of lipogenesis and adipogenesis.