Mitochondrial Dynamics and Mitochondria-Lysosome Contacts in Neurogenetic Diseases
Mitochondrial network is constantly in a dynamic and regulated balance of fusion and fission processes, which is known as mitochondrial dynamics. Mitochondria make physical contacts with almost every other membrane in the cell thus impacting cellular functions. Mutations in mitochondrial dynamics ge...
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Published in | Frontiers in neuroscience Vol. 16; p. 784880 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Research Foundation
31.01.2022
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Mitochondrial network is constantly in a dynamic and regulated balance of fusion and fission processes, which is known as mitochondrial dynamics. Mitochondria make physical contacts with almost every other membrane in the cell thus impacting cellular functions. Mutations in mitochondrial dynamics genes are known to cause neurogenetic diseases. To better understand the consequences on the cellular phenotype and pathophysiology of neurogenetic diseases associated with defective mitochondrial dynamics, we have compared the fibroblasts phenotypes of (i) patients carrying pathogenic variants in genes involved in mitochondrial dynamics such as
(also known as
),
,
, and
, and (ii) patients carrying mutated genes that their dysfunction affects mitochondria or induces a mitochondrial phenotype, but that are not directly involved in mitochondrial dynamic network, such as
(encoding frataxin, located in the mitochondrial matrix),
(hyperfission phenotype), and
(enlarged mitochondria phenotype). We identified mitochondrial network alterations in all patients' fibroblasts except for
. Functionally, all fibroblasts showed mitochondrial oxidative stress, without membrane potential abnormalities. The lysosomal area and distribution were abnormal in
,
,
, and
fibroblasts. These lysosomal alterations correlated with mitochondria-lysosome membrane contact sites (MCSs) defects in
exclusively. The study of mitochondrial contacts in all samples further revealed a significant decrease in
fibroblasts. GDAP1 and MFN2 are outer mitochondrial membrane (OMM) proteins and both are related to Charcot-Marie Tooth neuropathy. Here we identified their constitutive interaction as well as MFN2 interaction with LAMP-1. Therefore MFN2 is a new mitochondria-lysosome MCSs protein. Interestingly,
and
fibroblasts carry pathogenic changes that occur in their catalytic domains thus suggesting a functional role of GDAP1 and MFN2 in mitochondria-lysosome MCSs. Finally, we observed starvation-induced autophagy alterations in
,
,
,
, and
fibroblasts. These genes are related to mitochondrial membrane structure or lipid composition, which would associate the OMM with starvation-induced autophagy. In conclusion, the study of mitochondrial dynamics and mitochondria-lysosome axis in a group of patients with different neurogenetic diseases has deciphered common and unique cellular phenotypes of degrading and non-degrading pathways that shed light on pathophysiological events, new biomarkers and pharmacological targets for these disorders. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors share senior authorship This article was submitted to Neurogenomics, a section of the journal Frontiers in Neuroscience Edited by: Aurora Gomez-Duran, Margarita Salas Center for Biological Research, Spanish National Research Council (CSIC), Spain Reviewed by: Dario Ronchi, University of Milan, Italy; Marc Germain, Université du Québec à Trois-Rivières, Canada These authors have contributed equally to this work and share first authorship Present address: Arola Altimir, HIPRA Laboratories S.A., Girona, Spain Anna Altisent-Huguet, Reference Laboratory, Barcelona, Spain |
ISSN: | 1662-4548 1662-453X 1662-453X |
DOI: | 10.3389/fnins.2022.784880 |