A monophasic extraction strategy for the simultaneous lipidome analysis of polar and nonpolar retina lipids[S]

• Published in: Journal of lipid research Vol. 55; no. 8; pp. 1797 - 1809
• Main Authors: Lydic, Todd A., Busik, Julia V., Reid, Gavin E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2014; The American Society for Biochemistry and Molecular Biology; Elsevier
• Subjects: Animals; ganglioside; glycerolipid; lipid extraction; lipidomics; Lipids - chemistry; Lipids - classification; Lipids - isolation & purification; Liquid-Liquid Extraction - methods; Male; Mass Spectrometry; Methods; Rats; Retina - chemistry; sphingolipid; mass spectrometry; sphingolipid; lipid extraction; lipidomics; ganglioside; glycerolipid
• ISSN: 0022-2275; 1539-7262; 1539-7262
• DOI: 10.1194/jlr.D050302

Lipid extraction using a monophasic chloroform/methanol/water mixture, coupled with functional group selective derivatization and direct infusion nano-ESI-high-resolution/accurate MS, is shown to facilitate the simultaneous analysis of both highly polar and nonpolar lipids from a single retina lipid extract, including low abundance highly polar ganglioside lipids, nonpolar sphingolipids, and abundant glycerophospholipids. Quantitative comparison showed that the monophasic lipid extraction method yielded similar lipid distributions to those obtained from established “gold standard” biphasic lipid extraction methods known to enrich for either highly polar gangliosides or nonpolar lipids, respectively, with only modest relative ion suppression effects. This improved lipid extraction and analysis strategy therefore enables detailed lipidome analyses of lipid species across a broad range of polarities and abundances, from minimal amounts of biological samples and without need for multiple lipid class-specific extractions or chromatographic separation prior to analysis.