Functional Features of Alginates Recovered from Himanthalia elongata Using Subcritical Water Extraction

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 16; p. 4726
• Main Authors: Flórez-Fernández, Noelia, Domínguez, Herminia, Torres, María Dolores
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.08.2021; MDPI
• Subjects: Acetone; Algae; Alginates - chemistry; Alginates - isolation & purification; Alginates - pharmacology; Alginic acid; Anticoagulants; Antioxidants; antitumoral; autohydrolysis; biopolymer; Biopolymers; brown seaweed; Cell Line, Tumor; Cell lines; Cell Proliferation - drug effects; Cell Survival - drug effects; Cell viability; Elongation; Fourier transforms; Fractionation; Himanthalia elongata; Humans; Liquid phases; Metabolites; Molecular Conformation; NMR; Nuclear magnetic resonance; Ovaries; Phaeophyceae - chemistry; Rheological properties; Rheology; Size exclusion chromatography; Sodium; Sodium alginate; Spectroscopy, Fourier Transform Infrared; Temperature; Viscoelasticity; Water - chemistry; brown seaweed; biopolymer; antitumoral; rheology; autohydrolysis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26164726

Subcritical water extraction of and the subsequent acetone fractionation to precipitate crude fucoidans generated a liquid phase which was used to recover alginates with a wide range of viscoelastic features and other soluble extracts with potential biological activities. The precipitated alginate was converted to sodium alginate using an environmentally friendly treatment before being characterized by Fourier transform infrared attenuated total reflectance, nuclear magnetic resonance, high performance size exclusion chromatography and rheological measurements. The cell viability of three human cell lines (A549, HCT-116, T98G) in the presence of the extracts obtained before and after acetone fractionation was assessed. Fractionation with different acetone volumes showed a slight effect in the behavior of the different tested cell lines. Results also indicated a notable effect of the processing conditions on the block structure and molar mass of the extracted biopolymer, with the subsequent impact on the rheological properties of the corresponding gelled matrices.