Optimization of the Ultrasonic-Assisted Extraction Technology of Steroidal Saponins from Polygonatum kingianum Collett & Hemsl and Evaluating Its Quality Planted in Different Areas

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 5; p. 1463
• Main Authors: He, Sen, Wang, Xifu, Chen, Jiaqiang, Li, Xiaogang, Gu, Wen, Zhang, Fan, Cao, Guanhua, Yu, Jie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.02.2022; MDPI
• Subjects: Chromatography, High Pressure Liquid - methods; dioscin; diosgenin; Diosgenin - analogs & derivatives; Diosgenin - analysis; Diosgenin - chemistry; Diosgenin - isolation & purification; Drugs, Chinese Herbal - chemistry; Efficiency; Ethanol; Experiments; Herbal medicine; High performance liquid chromatography; Liquid chromatography; Methods; Optimization; Polygonatum; Polygonatum - chemistry; Polygonatum kingianum; Quality assessment; Response surface methodology; response surface methodology (RSM); Saponins; Saponins - analysis; Saponins - chemistry; Saponins - isolation & purification; Solvents; steroidal saponins; Steroids - analysis; Steroids - chemistry; Steroids - isolation & purification; Technology; Ultrasonic Waves; ultrasonic-assisted extraction (UAE); Ultrasonics; dioscin; Polygonatum kingianum; ultrasonic-assisted extraction (UAE); steroidal saponins; high-performance liquid chromatography (HPLC); diosgenin; response surface methodology (RSM)
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27051463

Collett & Hemsl is one of the famous traditional Chinese herbs with satisfactory therapeutic effects on invigorating Qi, nourishing Yin and moistening lungs, in which steroidal saponins are one class of important active substances. The main purpose is to determine the optimal extraction technology of steroidal saponins and evaluate the quality of planted in five different areas. The optimal ultrasonic-assisted extraction (UAE) technology was established by using single-factor experiments and the response surface methodology (RSM), and the determination method of high-performance liquid chromatography (HPLC) for dioscin and diosgenin, two primary types of acid-hydrolyzed steroidal saponins, was constructed with good linear range and precision. The results showed that UAE was an efficient extraction method for steroidal saponins, and the extraction yield was significantly affected by the liquid-solid ratio. The optimal extraction technology was generated following a liquid-solid ratio of 10:1 (mL/g), an ethanol concentration of 85% ( / ), an extraction time of 75 min, an extraction temperature of 50 °C and three extractions, of which these parameters were in line with the predicted values calculated by RSM. Considering only dioscin and diosgenin, the quality of planted at five sample plots presented non-significant difference. However, the content of diosgenin in Pingbian Prefecture (PB) was higher than that of the other four areas with a value of 0.46 mg/g. Taken together, the optimal UAE technology for steroidal saponins was determined via RSM. The quality evaluation revealed that there was a non-significant difference among planted in different areas based on the contents of the sum of dioscin and diosgenin. This work has important reference value for the exploitation and utilization of