Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 7; p. 1228
• Main Authors: Wang, Zhi-Peng, Wang, Qin-Qing, Liu, Song, Liu, Xiao-Fang, Yu, Xin-Jun, Jiang, Yun-Lin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.03.2019; MDPI
• Subjects: Amino acids; Biomass; By products; cane molasses; Carbon; Cell growth; Enzymes; Fermentation; isomaltulose; lipid; Lipids; Metabolites; Nitrogen; Sucrose; Syrups & sweeteners; Yarrowia lipolytica; Yeast; China; cane molasses; isomaltulose; lipid; Yarrowia lipolytica
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24071228

Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L−1) at flask level with pretreated cane molasses of 350 g L−1 and corn steep liquor of 1.0 g L−1. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L−1) was achieved with 0.96 g g−1 yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L−1). Additionally, the lipids comprised of 94.29% C16 and C18 fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.