Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery ( Apium graveolens L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 3; p. 698
• Main Authors: Emad, Ayat M, Rasheed, Dalia M, El-Kased, Reham F, El-Kersh, Dina M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.01.2022; MDPI
• Subjects: Acetic acid; Antiinfectives and antibacterials; antimicrobial; Antimicrobial activity; Antimicrobial agents; antioxidant; Antioxidants; Antioxidants - pharmacology; Apiaceae; Apium - enzymology; Apium - metabolism; Apium - physiology; Apium graveolens; Assaying; Butanol; Celery; Chelation; Chromatography, High Pressure Liquid - methods; Dichloromethane; E coli; Egypt; Ethyl acetate; Ferric ions; Flavonoids; Flavonoids - analysis; Fungicides; Herbal medicine; Iron constituents; Medicinal plants; Metabolism; Metabolites; Microbial Sensitivity Tests; Minimum inhibitory concentration; Organic solvents; Phenols - analysis; Phenylpropanoids; Picrates - chemistry; Plant Components, Aerial - chemistry; Plant extracts; Plant Extracts - pharmacology; Plants, Medicinal - drug effects; Polyphenols; Polyphenols - analysis; Public health; Scavenging; Spectrometry, Mass, Electrospray Ionization - methods; Sulfonic acid; Sulfonic Acids - analysis; UPLC/ESI/TOF-MS; Vitamin E; Egypt; UPLC/ESI/TOF-MS; antimicrobial; antioxidant; celery; Apium graveolens
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27030698

Medicinal plant extracts are increasingly considered a major source of innovative medications and healthcare products. This study focused on preparing a polyphenol enriched water extract of Egyptian celery " L., Apiaceae" aerial parts (TAE) in an endeavor to accentuate its antioxidant capacity as well as its antimicrobial activity. (TAE) of celery was partitioned against different organic solvents to yield dichloromethane (DCM), ethyl acetate (EAC), and butanol (BUOH) fractions. (TAE) and the organic fractions thereof besides the remaining mother liquor (ML) were all screened for their antioxidant capacity using various protocols viz. monitoring the reducing amplitudes for ferric ions (FRAP), and radical scavenging potentials of oxygen (ORAC), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelation assays. The examination procedure revealed both (TAE) extract and (DCM) fraction, to pertain the highest antioxidant potentials, where the IC of the (TAE) using ABTS and metal chelation assays were ca. 34.52 ± 3.25 and 246.6 ± 5.78 µg/mL, respectively. The (DCM) fraction recorded effective results using the FRAP, ORAC, and DPPH assays ca. 233.47 ± 15.14 and 1076 ± 25.73 μM Trolox equivalents/mg sample and an IC 474.4 ± 19.8 µg/mL, respectively. Additionally, both (TAE) and (DCM) fraction exerted antimicrobial activities recording inhibition zones (mm) (13.4 ± 1.5) and (12.0 ± 1.0) against and (11.0 ± 1.2) and (10.0 ± 1.3) against , respectively, with no anti-fungal activity. Minimum inhibitory concentration (MIC) of (TAE) and (DCM) fraction were 1250 and 2500 µg/mL, respectively. UPLC/ESI/TOF-MS unveiled the chemical profile of both (TAE) and (DCM) fraction to encompass a myriad of active polyphenolic constituents including phenylpropanoids, coumarins, apigenin, luteolin, and chrysoeriol conjugates.