Salvianolic acid Y: a new protector of PC12 cells against hydrogen peroxide-induced injury from Salvia officinalis

• Published in: Molecules (Basel, Switzerland) Vol. 20; no. 1; pp. 683 - 692
• Main Authors: Gong, Jun, Ju, Aichun, Zhou, Dazheng, Li, Dekun, Zhou, Wei, Geng, Wanli, Li, Bing, Li, Li, Liu, Yanjie, He, Ying, Song, Meizhen, Wang, Yunhua, Ye, Zhengliang, Lin, Ruichao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.01.2015; MDPI
• Subjects: Acids; Alkenes - chemistry; Alkenes - pharmacology; Animals; Benzofurans - chemistry; Benzofurans - pharmacology; Chinese medicine; circular dichroism (CD); Cytoprotection - drug effects; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Hydrogen Peroxide - toxicity; Neuroprotective Agents - chemistry; Neuroprotective Agents - pharmacology; PC12 Cells; Polyphenols - chemistry; Polyphenols - pharmacology; Proton Magnetic Resonance Spectroscopy; Rats; Salvia officinalis; Salvia officinalis - chemistry; Beijing China; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules20010683

Salvianolic acid Y (TSL 1), a new phenolic acid with the same planar structure as salvianolic acid B, was isolated from Salvia officinalis. The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods, including 1D, 2D-NMR (1H-1H COSY, HMQC and HMBC) and circular dichroism (CD) experiments. The biosynthesis pathway of salvianolic acid B and salvianolic acid Y (TSL 1) was proposed based on structural analysis. The protection of PC12 cells from injury induced by H2O2 was assessed in vitro using a cell viability assay. Salvianolic acid Y (TSL 1) protected cells from injury by 54.2%, which was significantly higher than salvianolic acid B (35.2%).