Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae

• Published in: The journal of microbiology Vol. 50; no. 5; pp. 882 - 887
• Main Authors: Naicker, Mahendran Chinnamara, Sejong University, Seoul, Republic of Korea, Jo, I.S., Sejong University, Seoul, Republic of Korea, Im, H.N., Sejong University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.10.2012; Springer Nature B.V; 한국미생물학회
• Subjects: Biomedical and Life Sciences; Cell cycle; chaperone; Chaperones; Chromosomes; Cold; cold shock response; Cold tolerance; Deletion mutant; Denaturation; E coli; freeze tolerance; Freeze-thawing; Freezing; Gene Expression Regulation, Fungal; Genes; Glycerol; Heat; Heat shock proteins; LEVADURA; LEVURE; Life Sciences; Low temperature; Microbiology; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; mutants; Protein denaturation; Protein folding; Protein interaction; proteins; Reintroduction; SACCHAROMYCES CEREVISIAE; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Stress; temperature; Temperature effects; Yeast; YEASTS; 생물학; cold shock response; chaperone; yeast; freeze tolerance
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-012-2411-z

Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.