Eight Years of Experience with Molecular Identification of Human Enteroviruses

We have successfully typed 1,121 human enterovirus (HEV) isolates during the last 8 years by adapting partial VP1 sequencing to routine identification of HEV isolated from diverse clinical and environmental specimens. The isolates include 48 of the 59 traditional nonpoliovirus HEV serotypes and memb...

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Published inJournal of Clinical Microbiology Vol. 46; no. 7; pp. 2410 - 2413
Main Authors Blomqvist, Soile, Paananen, Anja, Savolainen-Kopra, Carita, Hovi, Tapani, Roivainen, Merja
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.07.2008
American Society for Microbiology (ASM)
Subjects
Biological and medical sciences
Cluster Analysis
Enterovirus
Enterovirus - classification
Enterovirus - isolation & purification
Enterovirus Infections - virology
Environmental Microbiology
Epidemiology
Fundamental and applied biological sciences. Psychology
Genotype
Hepatitis E virus
Humans
Microbiology
Molecular Sequence Data
RNA, Viral - genetics
Sequence Analysis, DNA
Sequence Homology
Human
Microbiology
Identification
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Summary:We have successfully typed 1,121 human enterovirus (HEV) isolates during the last 8 years by adapting partial VP1 sequencing to routine identification of HEV isolated from diverse clinical and environmental specimens. The isolates include 48 of the 59 traditional nonpoliovirus HEV serotypes and members of 8 newly discovered types, which would have remained untypeable by neutralization using the conventional cross-sectional pools of antisera.
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Corresponding author. Mailing address: Enterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute (KTL), Mannerheimintie 166, 00300 Helsinki, Finland. Phone: 358 9 47448490. Fax: 358 9 4744 8355. E-mail: soile.blomqvist@ktl.fi
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00313-08